UAM-X

Antiurolithiatic activity of Eysenhardtia

polystachya aqueous extract on rats

R. M. PEREZ G., R. VARGAS S., S. PEREZ G. AND M. ZAVALA S.

Abstract

The aqueous extract of the bark of Eysenhardtia polystachya was tested for its antilithiatic and diuretic activity. The urolithiasis was induced experimentally by implantation of a zinc disc in to the urinary bladder of rats. A significant decrease in the weight of stones was observed after treatment in animals that received aqueous extract compared with control groups. This extract showed an increase in the 24 h urine volume compared with the control. # 1998 John Wiley & Sons, Ltd. Phytother. Res. 12, 144–145, (1998).

Keywords: Eysenhardtia polystachya; antilithiatic activity; diuretic activity

Antiurolithiatic-activity-of-Eysenhardtia-polystachya-aqueous-extract-on-rats.pdf

Cytotoxic Isoflavans from Eysenhardtia polystachya

LAURA ÁLVAREZ, MARÍA YOLANDA RÍOS, CARLOS ESQUIVEL, MARÍA ISABEL CHÁVEZ, GUILLERMO DELGADO, MARÍA ISABEL AGUILAR, MARÍA LUISA VILLARREAL AND VÍCTOR NAVARRO

Abstract

Two new cytotoxic isoflavans, (3S)-7-hydroxy-2’,3’,4’,5’,8-pentamethoxyisoflavan (1) and (3S)-3’,7-dihydroxy-2’,4’,5’,8-tetramethoxyisoflavan (2), were isolated from the bark and trunks of Eysenhardtia polystachya (Leguminosae), together with the known constituents stigmasterol, isoduartin, cuneatin, 7-hydroxy-2’,4’,5’-trimethoxyisoflavone, and 3,4-dimethoxy-8,9-(methylenedioxy) pterocarpan. The structures of 1 and 2 were elucidated on the basis of spectroscopic methods. The antimicrobial, cytotoxic, and insecticidal potential of some of these compounds were evaluated. The isoflavans 1, 2, and isoduartin (2’,7-dihydroxy-3’,4’,8-trimethoxyisoflavan) displayed moderate cytotoxic activity against KB cell lines.

Cytotoxic-Isoflavans-from-Eysenhardtia-polystachya.pdf

Development of a capillary electrophoresis method

for the characterization of “palo azul” (Eysenhardtia

polystachya)

Abstract

The tree Eysenhardtia polystachya (Ortega) Sarg. has quite a wide popular use within the traditional Mexican medicine as herbal remedy. Popular practices constitute a relevant enough basis to design optimum analytical methods in order to determine basic principles of diverse medicinal plants. This has become one of the essentials needed to characterize such products, for which it is fundamentally important to develop an efficient and reliable separation method. This work presents the results concerning the development and optimization of a novel CE method for the separation of components from water/etanol (1:1) extracts of E. polystachya, using the following conditions, considered the best obtained: phosphate buffer 10 mM, 20 kV voltage, and pH 8.1 at 214 nm and 50 mM, 12.5 kV voltage with pH 8.1 at 426 nm. The optimization takes into account the parameters associated in the resulting electropherograms, such as number of peaks, migration times, and the Dtm of the neighboring peaks. Under optimal conditions the separation intended was attained within 15 and 20 min for 214 and 426 nm, respectively. The characterization method developed was applied to the analysis of diverse extracts of E. polystachya.

Keywords: Characterization method / CZE / Eysenhardtia polystachya / Fingerprints / Mexican “palo azul”