Antioxidant and Anticancer Activities of Citrus reticulate (Petitgrain Mandarin) and
Pelargonium graveolens (Geranium) Essential Oils
SAYED A. FAYED
Abstract
The essential oils isolated by hydro-distillation from Pelargonium graveolens (geranium) and Citrus reticulate (petitgrain mandarin) were analyzed by GC/MS and assessed for antioxidant and anticancer activities. Twenty five components of petitgrain mandarin essential oil were identified and the major components were γ-terpinene (47.89%), methyl N-methyl anthranilate (13.17%), α-terpinene (7.40%), β-phellandrene (6.26%) and trans-isolimonene (5.87%). On the other hand, Thirty two compounds constituting 99.23% of geranium essential oil have been identified. The major components were citronellol (29.90%), trans-geraniol (18.03%), 10-epi-γ-eudesmol (8.27%), isomenthone (5.44%) and linalool (5.13%). The DPPH free radical scavenging activities of petitgrain mandarin and geranium essential oils at various concentrations ranging from 25 to 200 μg/ml were determined. The essential oils of both plants reduced the concentration of DPPH free radical with an efficacy near to that of standard antioxidant (ascorbic acid). 50% effective concentration (EC50) of petitgrain mandarin essential oil (79.84 μg/ml) was higher than geranium essential oil (66.45 μg/ml). It was indicated that the antioxidant activity of the geranium essential oil was higher than that of the petitgrain mandarin essential oil. In addition to the antioxidant activity, the anticancer activity of the two essential oils on two human promyelocytic leukemia cell lines (HL-60 and NB4) using trypan blue assay were investigated in vitro. HL-60 and NB4 cell lines were treated with the essential oils samples at different concentrations ranging from 25 to 200 μg/ml. Geranium essential oil showed the highest anticancer activity with the LC50 values of 62.50 μg/ml in NB4 cell line and 86.5 μg/ml in HL-60 cell line, whereas petitgrain mandarin essential oil gave the LC50 values of 85.05 μg/ml in NB4 cell line and 105.73 μg/ml in HL-60 cell line. The results demonstrated the potential of the two essential oils for cancer treatments.
Key words: Citrus reticulate, Pelargonium graveolens, Essential oils, antioxidant, anticancer.
Antioxidant-and-Anticancer-Activities-of-Pelargonium-graveolens.pdf
Assessment of Pelargonium graveolens
oil as plant-based antimicrobial and
aflatoxin suppressor in food preservation
PRIYANKA SINGH, BHAWANA SRIVASTAVA, ASHOK KUMAR, RAJESH KUMAR,
NAWAL K. DUBEY AND RAJESH GUPTA
Abstract
BACKGROUND: Contamination of stored food commodities by moulds and mycotoxins results in qualitative as well as quantitative losses. Most of the synthetic antimicrobials used for preservation of stored food items produce side effects in the form of residual and mammalian toxicity. Recently some higher plant products have been recommended as safe alternatives of such synthetic antimicrobials. In the present investigation antifungal efficacy of some essential oils was evaluated against two toxigenic strains of Aspergillus flavus with special reference to the oil of Pelargonium graveolens to investigate its potential to inhibit aflatoxin B1 secretion.
RESULTS: Essential oil of P. graveolens exhibited absolute fungitoxicity against both the toxigenic strains of A. flavus. The minimum inhibitory concentration of the oil was found to be 0.75 gL−1 and exhibited a fungistatic nature. It was found superior over the synthetic fungicides tested and exhibited a broad fungitoxic spectrum. The oil showed excellent anti-aflatoxigenic efficacy as it completely inhibited aflatoxin B1 production even at 0.50 gL−1.
CONCLUSION: This is the first report on the aflatoxin B1 inhibitory nature of P. graveolens oil. It may be recommended as a novel plant-based antimicrobial as well as aflatoxin B1 suppressor over synthetic preservatives in food protection.
Keywords: anti-aflatoxigenic; antifungal; Aspergillus flavus; Pelargonium graveolens
Assessment-of-Pelargonium-graveolens-oil.pdf
Nematicidal activity of essential oil of Pelargonium graveolens
Against the root-knot nematode Meloidogyne incognita
N. K, LEELA, R. Khan, P. P. REDDY AND E. S, J. NIDIRY
Abstract
Nematicidal activity of essential oil of Pelargonium graveolens L. (cv. Algerian) and its major constituents namely citronellol, geraniol and linalool was determined against the root-knot nematode Meloidogyne incognita. Geraniol was found to be the most effective constituent which was followed by citronellol and Iinalool.
NEMATICIDAL-ACTIVITY-OF-ESSENTIAL-OIL-OF-PELARGONIUM-GRAVEOLENS.pdf