Antinociceptive and anti-inflammatory effects of Tanacetum
parthenium L. extract in mice and rats
NAVEEN K. JAIN, SHRINIVAS K. KULKARNI
Abstract
Oral administration of the feverfew (Tanacetum parthenium) extract led to significant antinociceptive and anti-inflammatory effects against acetic acid-induced writhing in mice and carrageenan-induced paw edema in rats, respectively. These responses were dose-dependent (10, 20, 40 mg:kg, p.o.). Parthenolide (1, 2 mg:kg i.p.), the active constituent of the extract also produced antinociceptive and anti-inflammatory effects. Naloxone (1 mg:kg i.p.), an opiate antagonist, failed to reverse feverfew extract and parthenolide-induced antinociception. Feverfew extract in higher doses (40, 60 mg:kg p.o.) neither altered the locomotor activity nor potentiated the pentobarbitone-induced sleep time in mice. It also did not change the rectal temperature in rats. Feverfew extract exerted antinociceptive and anti-inflammatory effects without altering the normal behaviour of the animals. © 1999 Elsevier Science Ireland Ltd. All rights reserved.
Keywords: Feverfew extract; Parthenolide; Antinociception; Anti-inflammatory
Antinociceptive-feverfew5329e14cd1b20.pdf
Antioxidant constituents in feverfew (Tanacetum parthenium)
extract and their chromatographic quantification
CHANGQING WU, FENG CHEN, XI WANG, HYUN-JIN KIM, GUO-QING HE,
VIVIAN HALEY-ZITLIN, GEORGE HUANG
Abstract
Medicinal herb feverfew (Tanacetum parthenium) has been reported to possess prophylactic properties over migraine and arthritis. However, less attention has been given to its antioxidant activities. In our study the antioxidant activities of the feverfew extract and its bioactive components in terms of their free radical-scavenging activities against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and their Fe2+-chelating capacities were determined. In addition, the bioactive constituents in feverfew were determined by GC–MS and HPLC–UV. The results showed that feverfew powder extracted by 80% alcohol contained camphor, parthenolide, luteolin and apigenin in 0.30 ± 0.08%, 0.22% ± 0.03%, 0.84% ± 0.10% and 0.68% ± 0.07%, respectively. Total phenolic content of the feverfew extract was measured in 21.21 ± 2.11 lg gallic acid equivalent per mg dry material. The feverfew alcoholic extract possessed a strong DPPH free radical-scavenging activity of 84.4% and moderate Fe2+-chelating capacity of 53.1%. Luteolin also showed strong DPPH scavenging activity of approximately 80% at P 0.52 mg/mL. Parthenolide exhibited weak DPPH scavenging activity of 15% and moderate Fe2+-chelating capacity of nearly 60%. Similar moderate Fe2+-chelating activity (approximately 60%) was observed for luteolin and apigenin at 2 mg/mL. © 2005 Elsevier Ltd. All rights reserved.
Keywords: Parthenolide; Feverfew; Tanacetum parthenium; Flavonoid; Antioxidant activity; DPPH; Metal-chelating; HPLC; GC–MS
Antioxidant-constituents-in-feverfew5329e14d0385c.pdf
Feverfew (Tanacetum parthenium) as a
Prophylactic Treatment for Migraine:
A Double-blind Placebo-controlled Study
D. PALEVITCH, G. EARON AND R. CARASSO
Abstract
To assess the effectiveness of feverfew as a prophylactic therapy for migraine, a double-blind placebo controlled cross-over trial was conducted for a period of 4 months. Fifty seven patients who attended an outpatient pain clinic were selected at random and divided into two groups. Both groups were treated with feverfew in the preliminary phase (phase 1), which lasted 2 months. In the second and third phases, which continued for an additional 2 months, a double-blind placebo-controlled cross-over study was conducted. The results showed that feverfew caused a significant reduction in pain intensity compared with the placebo treatment. Moreover, a profound reduction was recorded concerning the severity of the typical symptoms that are usually linked to migraine attacks, such as vomiting, nausea, sensitivity to noise and sensitivity to light. Transferring the feverfew-treated group to the placebo treatment resulted in an augmentation of the pain intensity as well as an increase in the severity of the linked symptoms. In contrast, shifting the placebo group to feverfew therapy resulted in a reduction of the pain intensity as well as in the severity of the linked symptoms. © 1997 John Wiley & Sons, Ltd.
Keywords: Feverfew; Tanacetum parthenium; parthenolide; migraine; headache; double-blind placebo controlled study.
Feverfew-(Tanacetum-parthenium)-as-a-Prophylactic-Treatment-for-Migraine.pdf
The flavonoids of Tanacetum parthenium and T. vulgare and their
anti-inflammatory properties
CHRISTINE A. WILLIAMSA, JEFFREY B. HARBORNEA, HANS GEIGERB, J. ROBIN S. HOULT
Abstract
The lipophilic flavonoids in leaf and flower of Tanacetum parthenium and T. vulgaris have been compared. While those of T. parthenium are methyl ethers of the flavonols 6-hydroxykaempferol and quercetagetin, the surface flavonoids of T. vulgare are methyl ethers of the flavones scutellarein and 6-hydroxyluteolin. Apigenin and two flavone glucuronides are surprisingly present in glandular trichomes on the lower epidermis of the ray florets of T. parthenium. The opportunity has been taken to revise the structures of the four 6-hydroxy¯avonol methyl ethers of T. parthenium based on NMR measurements. These are now shown to be uniformly 6- rather than 7-O-methylated. Tanetin, previously thought to be a new structure, is now formulated as the known 6-hydroxykaempferol 3,6,4 '-trimethyl ether. The vacuolar flavonoids of both plants are dominated by the presence of apigenin and luteolin 7-glucuronides; nine other glycosides were present, including the uncommon 6-hydroxyluteolin 7-glucoside in T. vulgare. When the major flavonol and flavone methyl ethers of the two plants were tested pharmacologically, they variously inhibited the major pathways of arachidonate metabolism in leukocytes. There were significant differences in potency, with the tansy 6-hydroxy¯avones less active than the feverfew 6-hydroxy¯avonols as inhibitors of cyclo-oxygenase and 5-lipoxygenase. # 1999 Elsevier Science Ltd. All rights reserved.
Keywords: Tanacetum parthenium; T. vulgare; Compositae; Feverfew; Tansy; 6-Hydroxykaempferol 3,6,4'-trimethyl ether; 6-Hydroxyluteolin 6,7,4'-trimethyl ether; Thromboxane B2; Leukotriene B4; Anti-inflammatory activity
The-flavonoids-of-Tanacetum-parthenium-and-T.-vulgare-and-their-anti-inflammatory-properties5329e14cb84c4.pdf